This 150×, 1.45 NA oil objective facilitates total internal reflectance fluorescence (TIRF) research microscopy over the UV to IR spectrum. The high-NA Olympus U Apochromat objective enables TIRF observation using a standard coverglass and standard immersion oil. The objective offers UV transmission down to 330 nm and a high numerical aperture, making it possible to perform TIRF microscopy and UV applications such as un-caging and/or photo-activation with only one lens. In addition, the objective offers IR transmission beyond 1000 nm, making it suited to IR fluorescence imaging and IR laser applications, and opening up the potential for live-cell microscopy and future applications in the growing IR microscopy field. The high magnification of the objective enhances small specimen structures on the CCD camera. Due to a small field of view, the optic's illumination light is concentrated onto a very small area of the specimen, offering added illumination intensity useful in single molecule fluorescence applications. The objective offers evanescent wave penetration characteristics similar to the existing 60× NA 1.45 TIRF objective. It features a working distance of 0.10 and a field number of 22, and uses a correction collar to compensate for coverglass thickness and temperature.