MicroSuite FIVE is an upgraded version of the microscope imaging software produced by Olympus and its affiliate Soft Imaging System Corp. (Muenster, Germany and Lakewood, CO). The software is fully integrated with Olympus microscopes, including the BX61, IX81 and AX70 motorized microscopes, as well as third party stages and other devices. The product supports numerous CCD and video cameras and TWAIN devices. In addition to microscope control, the introductory level package features image acquisition, simple image processing, interactive measurements, and report and database integration. The Basic Edition builds upon these features with additional functionality, including multi-channel fluorescence acquisition, image stitching based on pattern recognition, extended focal imaging, macro-recording, and remote visualization of an image via an IP-addressable camera. Biological Suite and Analytical Suite provide further functionality, including automated particle analysis and stage navigation to acquire overview images. The Advanced Suite offers multidimensional time-lapse analysis, 3-D rendering, slide- or multi-well processing, and simple deconvolution. Modules can be added to increase the functionality of each package. NetCam is an exciting new feature included in MicroSuite FIVE Basic Edition and higher level packages. Designed for use with the Olympus DP70 and DP30 cameras and the Soft Imaging System FireWire cameras (FVII, CC12, CVII, and CVIII), NetCam uses TCP/IP to broadcast a live image over the Internet via an assigned static IP address.