Olympus America Inc.
Scientific Equipment Group
3500 Corporate Pkwy.
Center Valley, PA, 18034
Website: http://www.olympusamerica.com/microscopes
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Confocal Live Cell Imaging
The Olympus FluoView™ FV1000 confocal microscope features a SIM Scanner, which incorporates two independent, fully synchronized laser scanners in a compact design for simultaneous laser stimulation and confocal observation. Rapid cellular reactions that occur during or immediately following laser stimulation may be captured without lag time. This instrument finds use in techniques such as FRAP, FLIP, uncaging, photoactivation, and photoconversion. Optical design provides 2-nanometer (nm) wavelength resolution, and high-speed spectroscopy at 100nm/msec. Two independent photomultiplier tube (PMT) channels for spectral detection are each configured with a diffraction grating and variable slit for high-resolution wavelength separation and high-speed bandwidth selection. Normal and Blind spectral unmixing modes are provided for automatic discrimination between fluorochromes of similar color. The PMTs, selected for high efficiency, can be used in either analog accumulation (AAC) mode or in hybrid photon counting mode (HPCM), for low light specimens. Ion deposition filters are used for increased sensitivity and full wavelength coverage. High signal-to-noise ratios ensure efficient fluorescence detection even under low laser conditions that minimize damage to living cells. Scan speed is 16 frames per second with 1ms/100nm wavelength scans. Laser feedback and stabilizing system provides precise control of the laser intensity over time, a necessary feature for accurate time-lapse quantitation.
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