Cheminert Nanovolume HPLC injectors and switching valves facilitate high resolution HPLC at a small scale. The 100 μm bore is uniform throughout the flow path, reducing band broadening and creating less than 25 nl of port-to-port volume. New individual finger-tight fittings work with a variety of tubing, including PEEK, fused silica (360 μm) and 1/32 electroformed nickel. The fast (100 msec) microelectric actuator determines accurate positioning without limit switches or customer adjustment. Computer control is facilitated via RS-232 or RS-485 interface.