Used in screening for high producing clones or quantifying antibody levels for production, the Guava RapidQuant Human IgG Kit offers efficient antibody quantitation: no washing steps, no dilution of typical hybridoma supernatants, no degradation of colorimetric readouts, no need to worry about assaying for each human IgG subtype. Available for both human and mouse IgG, the kits are bead-based for quick assessment of antibody concentrations in supernatant. Antibodies from hybridoma supernatant are captured on beads where they are detected by fluorescently-labeled detector antibodies. The fluorescence intensity of the beads in each sample is proportional to the antibody concentration of the supernatant. Using a standard curve generated with the provided human IgG standard allows determination of absolute antibody concentration.