Guava Technologies, Inc.
25801 Industrial Blvd.
Hayward, CA, 94545
Website: http://www.guavatechnologies.com
|
 |
Cell Cycle Assay
Cell Cycle Assay (Guava Technologies, Inc.) incorporates staining reagents pre-combined in a single optimized format, eliminating reagent preparation following cell fixation. Protocols are offered for use with either individual tubes or 96-well microplates, minimizing assay development time. Automated analysis capability displays results in both histogram and statistical formats, making it a convenient method of screening and optimizing lead drug candidates for their ability to induce cell cycle arrest and to kill proliferating cells. With Guava's Personal Cell Analysis system and CellHealth Profiling assays, the assay provides benchtop cell analysis capabilities. The cell cycle assay uses the nuclear DNA stain propidium iodide (PI) to directly discriminate cells at different stages of the cell cycle. Incubation can be as short as only one hour. Three histogram markers distinguish the G0/G1, S and G2/M cell cycle phases, while an optional fourth marker offers a way to identify apoptotic cells, cell aggregates or an internal standard. Absolute cell counts are provided automatically. Absolute counts can enable researchers to determine whether changes in cell cycle and other functional parameters and biomarker expression are a result of drug treatments or are caused by changes in cell number due to cell proliferation or cell death.
|
|
