Marker Gene Technologies Inc.
1850 Millrace Drive
Eugene, OR, 97403
Website: http://www.markergene.com
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Apoptosis Detection Kits
Magic Red™ substrate-based assays detect protease activity within whole living cells, and are based on a cresyl violet leaving group that fluoresces once enzyme specific peptides are cleaved. FAM FLICA™ Apoptosis Detection Kits use a methodology based on cell-permeable and non-cytotoxic reagents called Fluorochrome Inhibitor of Caspases (FLICA). Once inside the cell, FLICA inhibitor binds covalently to the active caspase. For the FAM FLICA kits, which fluoresce green, a carboxyfluorescein-labeled fluoromethyl ketone peptide inhibitor of the specific caspase is used (EX: 488 nm/EM:520 nm), compatible with most fluorescence microscopes or microplate readers. SR-FLICA™ Apoptosis Detection Kits use a methodology based on a cell-permeable and non-cytotoxic FLICA. Once inside the cell, the FLICA inhibitor binds covalently to the active caspase. For the SR FLICA kits, which fluoresce red, a sulforhodamine-labeled fluoromethyl ketone peptide inhibitor is used.
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