GIBCO media bags have universal tubing connections for easy integration into automated cell maintenance systems. The bags are pre-filled with media in a variety of formulations and sizes and are stocked by the manufacturer. Specially formulated media can be ordered in the universal bag as well. Four-port design offers compatibility with four different mechanical connection methods — blood spike, male-threaded luer, female-threaded luer, and MPC quick connect — and helps maintain a closed media transfer system by using three pre-connected C-Flex tubing transfer lines (40 in length). Flexibility in flow rates and uses is provided through the use of a variety of tubing sizes — 1/8× ¼, 3/16× 5/16, and 3/8× 5/8 (ID × OD). The bags are compatible with the most commonly used thermoplastic tubing for sterile and aseptic connection devices.