SuperSAP shrimp alkaline phosphatase formulation provides quick DNA dephosphorylation. The product removes 5' phosphates from protruding, blunt or recessed ends in just 5 minutes at 37 C. It works directly in restriction enzyme digests — just add directly to simultaneously cut and dephosphorylate DNA. No additional buffers or additives are required. SuperSAP is100% heat-inactivated at 65 C. Purification is not necessary prior to ligation.