USB Corporation
26111 Miles Road
Cleveland, OH, 44128
Website: http://www.usbweb.com
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Polymerase Mix
RubyTaq PCR Master Mix is supplied as a 2× pre-mixed formulation containing Taq DNA polymerase, ultrapure nucleotides, reaction buffer, and two inert dyes optimized for a wide variety of PCR applications. Neither loading dyes nor compounds that increase sample density need to be added prior to gel electrophoresis. During gel electrophoresis, RubyTaq separates into 2 colors, magenta and yellow, for easy visualization (Fig. 1). The magenta dye runs in a range between 500 bp (2% gels) and 1500 bp (0.8% gels) and the yellow dye runs at less than 10 bp. The dyes do not effect the migration rates of DNA and have no effect on the following procedures: PCR with co-solvents (e.g., betaine and DMSO); restriction digestion; automated or manual DNA sequencing; ligation-based or topoisomerase-based (TOPO) TA cloning; ExoSAP-IT PCR Clean-Up. If the dyes must be removed prior to another application (e.g., in vitro transcription), use phenol/chloroform extraction and ethanol precipitation or a commercially available dye removal purification product.
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