BioVision, Inc.
980 Linda Vista Ave.
Mountain View, CA, 94043-1903
Website: http://www.biovisionlabs.com
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HAT Activity Assay Kit
Histone acetyltransferases (HATs) play a crucial role in a variety of cellular functions such as gene transcription, differentiation, and proliferation. This HAT assay kit utilizes active recombinant HAT protein as a positive control and acetyl-CoA as a cofactor. Acetylation of peptide substrate by active HAT produces the free form of CoA that then serves as an essential coenzyme for producing NADH. NADH is easily detected spectrophotometrically after reacting with a tetrazolium dye. This kit provides a straightforward protocol for a complete assay in one to two hours.
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