Roche Applied Science

9115 Hague Rd.
P.O. Box 50414
Indianapolis, IN, 46250-0414
Website: http://www.roche.applied.science.com





DNA Dephosphorylation And Ligation Kit



Rapid DNA Dephos & Ligation Kit provides dephosphorylation and ligation of sticky- or blunt-end DNA fragments, enabling ligation of DNA in 5 minutes and dephosphorylation in 10 minutes. The kit contains the new recombinant rAPid Alkaline Phosphatase, which is easily heat-inactivated in 2 minutes at 75 C, eliminating phenol-chloroform extraction steps and allowing dephosphorylation to be performed directly in restriction enzyme digests. Procedures are thus simplified for a broad array of applications, including cloning of fragments into plasmid or phage vectors, linker ligation, recircularization of linear vector DNA, and generation of libraries.


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