These Miniblotters enable users to incubate different antibodies with antigens run in separate gel lanes and transferred to a membrane. In practice, an intact blotted membrane is mounted directly in the unit and the immunoassay is run in the channels. The instrument’s design creates discrete parallel channels on the membrane, which act as independent incubation chambers. The MN18 and MN26 have 18 and 26 channels, respectively, which match the lanes in BioRad’s Criterion pre-cast gels. Each chamber in the MN18 and MN26 holds sample volumes from 100 to 340 microliters. A sealing system ensures that there is no cross-channel contamination.