USA Scientific, Inc.
P.O. Box 3565
Ocala, FL, 34478
Website: http://www.usascientific.com
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Comparative Investigations On The Blocking Efficiency Of Filter Pipet Tips
Woody Moser
Introduction
The blocking efficiency of filter tips is investigated using airborne particles. Filter tips seated on air displacement pipettors are used to prevent potential contamination of the pipettor tip cones (i.e. DNA fragments). The more effective the filter is, the more risk of contamination is reduced. This is of utmost importance for preventing false-positive results, especially in sensitive PCR experiments. In this investigation, TipOne 200 l filter tips have been compared with those of other manufacturers.
Double-stranded DNA, i.e. the main product of PCR, is very long-lived, and one central issue for the laboratory using PCR procedures has to be the effective avoidance of contamination. It seems obvious that in the PCR procedure, pipetting of the PCR product (DNA) is one of the most sensitive steps. Cross-contamination occurs if improper pipetting causes splashes or drips. Even if pipettors are handled properly, aerosols can be generated that contain DNA molecules and can contaminate the pipettor and subsequent pipet products. This demands increasing efforts to reduce the penetration of contaminants by means of filter tips.
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