In-Fusion 2.0 PCR cloning system allows cloning into standard vectors, at any restriction site, with ease and efficiency. Precise clones can be generated without PCR product purification, restriction digestion, or blunt-end polishing, and the cloning is ligation-independent. The method relies on direct treatment of PCR products with Cloning Enhancer, and with the In-Fusion Enzyme, a protein that can fuse linear DNA with small regions of overlapping sequence efficiently and precisely. The simple reaction generates the desired clones in just two days. Individual tubes of lyophilized dry-down reagents assist in quick and easy cloning, and can be stored on the lab shelf.