The EN III Microbiology System performs identification of more than 1,000 species of gram negative and gram positive bacteria using a single test panel. No Gram-stain, pre-tests or follow-on tests are required, and set-up consists of a simple, one-minute protocol. A single 96-well microplate panel incorporates 71 carbon source and 23 chemical sensitivity assays in a pre-coated dry chemistry format. With these 94 tests, the system analyzes a bacterial cell’s properties, including its ability to metabolize all major classes of biochemicals and its sensitivity to chemicals that may inhibit growth. The colorimetric pattern or “fingerprint” that is generated by the organism is interpreted and compared against an extensive on-board library to produce a demonstrated level of