Animal-Free Blocker (5×) is a plant-derived blocking agent or diluent for nucleic acid or protein blotting applications. The reagent contains no proteins of animal origin. It can be used as an alternative to sera, BSA, casein or non-fat dry milk to avoid potential interaction between the immunoglobulin in these protein solutions and antibodies used for detection, especially anti-goat Ig or anti-sheep Ig. The immunoglobulins that may be significant in these commonly used blocking agents/diluents can bind to the detection antibodies and either reduce the signal or produce high background. Supplied as a 250 ml concentrate, it produces 1250 ml of working solution.
