FACSDiva 6.0 (BD Biosciences, San Jose, CA) will help scientists attain consistent data from flow cytometry systems used for basic and clinical research. The software includes Cytometer Setup and Tracking capability. This set of features characterizes and setup of the FACSCanto, FACSCanto II, LSR II, and FACSAria flow cytometry systems. Benefits include: data reproducibility; simplified design of multi-color experiments; yield of high quality data from multi-color experiments; and prompt identification of cytometer performance issues.