MP Biomedicals LLC
3 Hutton Centre Drive
Suite 100
Santa Ana, CA, 92707-8757
Website: http://www.qbiogene.com
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Purify mRNA From Cells, Tissue, Or Total RNA
New mTRAP mRNA Purification Kits incorporate a patent-pending peptide nucleic acid (PNA) molecular probe that has high binding affinity for mRNA. As a result, mRNA isolation can be performed in buffers with low salt concentrations, eliminating secondary structure that can make mRNA inaccessible for capture as well as non-specific bonds that can cause unwanted rRNA and protein contamination. mRNA can be selectively eluted away from unwanted genomic DNA, and mRNA with short poly A tails is made more available for capture. Kits are available in two sizes for purification directly from tissue or cells and in one size for the purification of mRNA from total RNA. The 24-prep mTRAP Midi Kit typically yields 10-20 μg of mRNA from 0.5-1.0 × 107 cells or 50-200 mg tissue while the 6-prep mTRAP Maxi Kit typically yields 100-200 μg of mRNA from 0.5-2.0 × 108 cells or 0.4-1.0 gram tissue. mRNA yields from the 12-prep mTRAP Total Kit are typically 10-15 μg from each 500 μg of total RNA. All three kits include all required plasticware and RNase-free solutions.
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