NativePure Native Protein Complex Purification and Analysis System enables the isolation of protein complexes, with analysis by native gel electrophoresis. pcDNA Gateway Vectors incorporate the BioEase tag, a 72-amino acid sequence from K. pneumoniae that directs in vivo biotinylation of a specific lysine residue. Biotin interacts strongly with streptavidin and avidin. Biotinylated proteins and protein complexes remain bound, as impurities are removed by thorough washing of the Streptavidin Agarose column. The vectors include 2 tandem AcTEV Protease recognition sites to release the bound biotinylated proteins and protein complexes from Streptavidin Agarose, creating a useful strategy for protein purification.