The miRacULS miRNA Isolation and Labeling Kit combines the upstream isolation of small RNA from tissue or cultured cells and the reagents necessary to label these samples for DNA microarray analysis. The kit is available with the ULS labeling molecule bound to Cy3 and Cy5 dyes licensed from GE Healthcare, and isolates and labels miRNAs as well as siRNAs. A 30 minute column-based isolation is followed by one-step, 15-minute, direct labeling without the need for enzymes. This kit also includes KREApure purification columns, which remove unreacted ULS reagent from the labeling reaction. The columns have no affinity for nucleic acids: recovery is high as fragments of all sizes flow through, making it suitable for work with miRNA samples.
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