The miRNA QRT-PCR detection kit uses a proprietary miRNA primer design approach to detect mature miRNA from as little as 15 ng of input, down to 100 copies, in less than three hours. Optimized QPCR reagents provide single nucleotide discrimination within and among miRNA families. MicroRNAs (miRNAs) are small, non-coding RNA molecules that are estimated to regulate at least 30% of all protein encoding genes. miRNAs have been implicated in development, differentiation, apoptosis, viral infection, and cancer. miRNAs are difficult to analyze by traditional PCR approaches due to their small size of approximately 22 nucleotides.
