Human SpliceMiner cDNA Library Panel simplifies the process of discovering rare and unique alternative splice forms. The panel consists of ~4.6 million primary cDNA clones derived from 15 cDNA plasmid libraries representing 28 cancer cell lines and 32 normal human tissues, including 14 sub-regions of the brain. Panel design facilitates capture of rare mRNAs and is enriched for complete cDNA transcripts, while maintaining mRNA representation found in the original human source material. SpliceMiner cDNA clones are arrayed into 384 lower complexity pools (4 × 96 well format). The pools are segregated to enhance the probability of amplifying one specific splice variant per well. The segregated pool in each well contains ~12,000 clones. Amplifying multiple lower-complexity pools increases the statistical probability of finding a single splice variant of a particular mRNA, especially for low-to-medium abundance transcripts.
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