QIAGEN Inc.

27220 Turnberry Ln.
Valencia, CA, 91355-1018
Website: http://www.qiagen.com





One-step RT-PCR



QuantiFast Probe RT-PCR Kits deliver fast results in real-time one-step RT-PCR with sequence-specific probes, such as TaqMan probes. A fast RT-PCR buffer containing the additive Q-Bond reduces denaturation, annealing, and extension times. The buffer also contains HotStarTaq Plus DNA Polymerase, which requires only five minutes at 95 C for activation. The optimized QuantiFast RT Mix enables cDNA synthesis in just 10 minutes. Accurate quantification of even low copy numbers is achieved with the kits. A balanced combination of KCl and NH4Cl in the fast RT-PCR buffer promotes specific primer annealing, enabling high PCR specificity and sensitivity. The product provides a stringent hot start, preventing the formation of nonspecific products.


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