QIAGEN Inc.
27220 Turnberry Ln.
Valencia, CA, 91355-1018
Website: http://www.qiagen.com
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Automated Plasmid DNA Purification
BioRobot 3000 workstation and DirectPrep 96 BioRobot Kit together provide an automated package for performing plasmid minipreps in a 96-well format. The procedure enables reproducible purification of plasmid DNA that is ready for direct use in high-throughput sequencing applications. The kit integrates lysis chemistry, eliminating the need for a lysate-clearing step, with an easily automated one-plate procedure. The integrated system enables purification of sequencing grade plasmid DNA from 96 samples per run. Typically, yields of up to 4 μg high-copy plasmid DNA can be obtained from 1.25 ml E. coli culture grown in LB medium. The yield of plasmid DNA is sufficient for several sequencing reactions and additional archiving of the sample.
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