Idaho Technology Inc.
Sales and Marketing
390 Wakara Way
Salt Lake City, UT, 84108
Website: http://www.idahotech.com/
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Melter, Dye, For Genotyping
Platform for post-amplification genotyping, gene scanning, and SNP/mutation detection uses closed tube DNA amplification reaction with the Idaho Technologies LCGreen I™ Dye and HR-1™ High Resolution Melter. Users are able to detect subtle differences in fluorescent signal over temperature change between different DNA species. Bringing scanning efforts to the bench reduces sequencing efforts and cost. The HR-1 System utilizes Roche LightCycler glass capillary tubes in an aluminum thermal cylinder using single peak excitation (440-470 nm) and emission (470-520 nm) spectra. The fluorescent dye is included in the amplification reaction and analysis is performed in a closed tube system. HR-1, in combination with LCGreen I, generates melting curves that exploit the subtle differences between heteroduplex and homozygous wild type species. The instrument can run 40-45 samples per hour (post amplification) with a 0.3 C/sec ramp.
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