Illumina TotalPrep RNA Amplification Kit is a complete system for generating biotinylated, amplified RNA for hybridization with Illumina Sentrix arrays. RNA amplification has become the standard method for preparing RNA samples for array analysis. The kit in vitro transcription reaction (IVT) has been optimized for the most effective biotin labeling. An NTP mix containing biotinylated UTP has been added to the kit, allowing ease of use and optimal labeling. These modifications increase sensitivity, providing increased Percent Present calls and a single round of amplification with just 50 ng of input RNA. A biotinylated NTP is not included in the kit. Ambion 800-888-8804 www.ambion.com