Sigma-Aldrich
3050 Spruce St
St Louis, MO, 63103
Website: http://www.sigma-aldrich.com/equipment
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Lentivirus-based RNAi Screening
MISSION LentiExpress shRNA-based system for high-throughput RNAi screening eliminates reagent preparation, utilizing a simple method to perform complex screens. An Optimization Plate enables quick optimization of the system for a particular cell line. A researcher simply adds the desired number of cells to each well, continues with optional selection and/or addition of small molecules (for example, pharmaceutical compounds), and then proceeds directly to the desired assay for gene silencing or loss-of-function. The LentiExpress format with the Human Kinase shRNA collection of The RNAi Consortium (TRC) enables rapid human kinome RNAi screening. The MISSION LentiExpress Human Kinase shRNA set consists of 3,109 individual pre-arrayed lentiviral clones harboring shRNA sequences that target 673 human kinase genes for gene silencing. The LentiExpress technology complements the MISSION shRNA product line, which includes libraries, gene family sets, and gene target sets for The RNAi Consortium lentiviral-based shRNA collection targeting the human and mouse genomes.
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