PhosphoProfile I Phosphopeptide Enrichment Kit features technology based on a novel Ga+3 chelate on silica that provides a robust method for phosphoproteomics. This approach offers very low non-specific binding, no bias in selectivity of phosphorylation state, high sensitivity, and fast handling, especially for small precious samples. Phosphoproteins are digested with proteomics-grade trypsin, resulting in quality phosphopeptides that are captured in the convenient spin-tubes. Recovered phosphopeptides are ready for analysis by either MALDI-TOF or ESI mass spectrometry. Up to 25 nanomoles of phosphopeptide can be captured and recovered from complex samples. Also included are the necessary buffers and mono and tetra phosphopeptide controls commonly used to verify performance eliminating the requirement of additional purchases and risk of data misinterpretation.