The Panorama Human Signal Transduction Protein Array contains 259 full-length proteins that are involved in cellular processes including proliferation, differentiation and apoptosis. The array employs a fusion tag that has been shown to confer protein functionality and that also serves to select, tether and uniformly orient proteins on a streptavidin-coated glass slide. This technology allows proteins to behave in a sterically unencumbered manner, thereby increasing the potential for acquiring data that is more closely representative of native physiological states.