EPICENTRE Biotechnologies
726 Post Rd.
Madison, WI, 53713
Website: http://www.epibio.com
|
 |
Purify RNA From A Single Cell
ArrayPure nano-scale RNA purification kit provides all reagents needed to purify RNA from one to ten thousand eukaryotic cells, as obtained with laser capture methods. The kit contains only aqueous solutions and requires no toxic organic solvents. This nano-scale protocol has been developed and tested with quantitative real-time PCR on 1 to 10,000 eukaryotic cells. Cell numbers were based on counted, then diluted, living cells. For very low cell numbers, cells were trapped in a microcapillary pipet and counted using an inverted microscope. After 2 rounds of RNA amplification, ArrayPure RNA from 20 HeLa cells produced microgram quantities of RNA.
|
|
