Promega Corporation
Communications
2800 Woods Hollow Rd.
Madison, WI, 53711
Website: http://www.promega.com
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Homogeneous Caspase 8 & 9 Assays
Caspase-Glo 8 and Caspase-Glo 9 assays offer sensitive apoptosis detection. The homogeneous assay protocol for Caspase 8 & 9 provides results in 20 minutes. Sensitivity is orders of magnitude greater than fluorescent methods, and the assays enable monitoring of toxic effects of compounds at high throughput or one sample at a time. The protocol consists of a single-step-reagent addition of a lysis/activity buffer containing a peptide-aminoluciferin caspase substrate and a thermostable luciferase. In the presence of caspase, the peptide is cleaved, releasing aminoluciferin for use by luciferase, which results in a "glow-type" luminescent signal that is proportional to caspase activity. The buffer allows rapid and efficient cell lysis while supporting optimal caspase and luciferase activities, and eliminates sample extract preparation steps.
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