PPAR Transcription Factor Assay provides a non-radioactive method for detecting specific transcription factor DNA binding activity in nuclear extracts. A specific double-stranded DNA (dsDNA) sequence containing the peroxisome proliferator response element (PPAR) is immobilized on a 96-well plate. PPARs contained in a nuclear extract bind specifically to the PPRE and are detected by a primary antibody directed against the specific PPAR. A secondary antibody conjugated to HRP is added to provide a sensitive colorimetric readout at 450 nm.

Cayman Chemical Co.
800-364-9897
www.caymanchem.com