Chemiluminescent Western blotting kits detect either rabbit or mouse antibodies complexed with antigen immobilized on PVDF or nitrocellulose membranes. The purified secondary antibody conjugates and matching reagents provide sensitivity, minimize species cross reactivity, and reduce lot-to-lot variation. All solutions required for blocking and washing the membrane and for diluting antibodies are supplied. The chemiluminescent substrate is provided as a stable two-component solution that emits light when combined with a horseradish peroxidase (HRP) or alkaline phosphatase (AP) labeled antibody. Results are recorded on X-ray film. AMRESCO Inc. 800-448-4442 www.amresco-inc.com