EnVision Multilabel Plate Readers (PerkinElmer Corp., Waltham, MA) are now available with both filter and monochromator technologies in a single system. Detection method can be chosen on the fly. Assays showing wide emission spectra are best captured with optimized filter bandwidths. EnVision’s user-changeable, label-specific optical mirror modules and filters provide detection sensitivity and measurement speed. Fast filter switching and real-time dispensing are offered for kinetic dual excitation/emission ion channel applications. Monochromator Options enable wavelength scanning and detection of fluorophores, even with small stokes shifts. The Absorbance Monochromator measures absorbance assays smoothly with a single monochromator while the Fluorescence Intensity Monochromator measures absorbance and fluorescence intensity with striking performance, using a pair of double grating monochromators.