Nanoprobes Inc.
95 Horse Block Rd.
Unit 1
Yaphank, NY, 11980-9710
Website: http://www.nanoprobes.com
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His-Tagged Protein Detection
Ni-NTA-Nanogold® detects and localizes His-tagged proteins using electron microscopy, light microscopy, and blotting. It features a 1.8-nm Nanogold particle with multiple nickel (II)-nitrilotriacetic acid (NTA) functionalities incorporated into the ligands on the surface of gold particles. Nickel-nitrilotriacetic acid functionalities bind to histidines from the tagged proteins and form stable complexes with extremely low dissociation constants. Using Ni-NTA-Nanogold, His-tagged fusion proteins originating from any of a variety of expression vectors can be labeled under both non-denaturing and denaturing conditions. These labeled proteins are visualized in the electron or light microscope and on blots when used with gold or silver enhancement reagents.
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