The LiveScan swept field confocal microscope (SFC), with NIS-Elements control and image acquisition software enables live cell confocal imaging. Pinhole scan mode provides high Z-axis resolution with data acquisition at rates up to 30 frames/sec, and in slit scan mode at acquisition frequencies up to 1200 frames/sec. The microscope monitors and records events with msec time resolution in living cells without compromising spatial confocality, while simultaneously minimizing specimen exposure to illumination energy that can result in photobleaching and phototoxicity. In pinhole mode, illuminating photons are focused through a row of 32 stationary pinholes over the specimen, allowing each point in the specimen to be illuminated up to 260 times/sec. The plate also contains two different size slits that allow fast data acquisition. This feature enables users to trade back and forth between scanning apertures to obtain an advantageous balance between speed, resolution and sensitivity for each experiment. Up to six acousto optical tunable filter (AOTF) modulated laser lines are available for single or multi-channel sequential data acquisition.
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