Spectral Imaging System - Bioscience Technology Online

Nikon Instruments Inc.

1300 Walt Whitman Rd.
Melville, NY, 11747
Website: http://www.nikoninstruments.com

Spectral Imaging System

DIGITAL ECLIPSE C1si confocal laser fluorescence microscope system can simultaneously acquire fluorescence spectra over a 320 nm range in wavelength at 10 nm spectral resolution, or over smaller ranges at 5 nm or 2.5 nm spectral resolution. When observed with a light microscope, fluorescent proteins are widely viewed as reporters of transcription in live cells. Acquisition of fluorescence spectra at high resolution mathematically separates the signals from each probe, and assigns it to a discrete data channel free from spillover. The result is a "stack" of images of the same object or scene, each at a different spectral narrow band or color. Emission path can easily be switched between standard three channel fluorescence detector and high resolution spectral detector. C1si sensitivity and signal-to-noise ratio make possible the high data acquisition rates necessary for live cell spectral imaging. Signals of fluorescent proteins can be cleanly separated, including CFP, GFP, YFP and DsRed, even in combination with closely overlapping antibody conjugates such as Alexa488, Cy3, and others. Clean separation of the signals of probes from autofluorescence is also possible.