HiTrap Butyl-S FF (1 ml and 5 ml) prepacked columns (GE Healthcare) enable purification or removal of highly hydrophobic proteins. HiTrap Butyl-S FF 1 ml column is included in the updated HiTrap HIC Selection Kit, which has been expanded to six hydrophobic interaction (HIC) media with different selectivities for small-scale media screening and purification. HiTrap Butyl-S FF is prepacked with Butyl-S Sepharose 6 Fast Flow, a hydrophobic chromatographic medium designed for purification of relatively strong hydrophobic molecules at low salt concentrations. The matrix Butyl-S Sepharose 6 Fast Flow is based on cross-linked agarose with a butyl ligand containing a sulfur atom, which means the ligand density can be accurately measured to secure reproducible production of the media. With a flexible design, HiTrap Butyl-S FF can be used with a syringe, a simple laboratory pump or a chromatography system, such as GE Healhcare's ÅKTAdesign or FPLC systems. GE Healthcare
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