New Brunswick Scientific
P.O. Box 4005
Edison, NJ, 08818-4005
Website: http://www.nbsc.com/bp6.htm
|
 |
Automated Yeast Cell Counting
NucleoCounter YC-100 automatically counts yeast cells in about 30 seconds. The system uses a fluorescence microscopy technique for accurately counting cell nuclei stained with propidium iodide. Combined with a CCD camera and integrated image analysis, the compact microscope facilitates fast and automatic cell counts. In order to determine the total concentration of yeast cells, the cells are treated with a lysis/disaggregation buffer which permeates the yeast cell wall and membranes, thereby allowing the cellular DNA to be stained with propidium iodide, which is safely immobilized on the inside of a disposable NucleoCassette. The reagent has the ability to dissociate minor cell clusters of yeast cells. Monitoring the cell number allows the scientist to activate protein expression systems or harvest at optimal times for optimal product yield. The system is pc-compatible for data export.
|
|
