Quick Blunting Kit provides a convenient method for preparing sheared, nebulized or restriction enzyme-digested DNA for blunt-ended ligation into a plasmid, cosmid, fosmid or BAC vector. In addition, it can also be used to prepare PCR products generated using non-phosphorylated primers for efficient blunt-end cloning. Fast- Restriction enzyme-digested DNA blunted is ready in less than 30 minutes. Reactions are performed at room temperature in a ready-to-use mix.