Supporting suspension and adherent cell growth and differentiation, both 24-well plate and single-well inserts for cell culture assays incorporate track-etched thin film membranes for reliable monolayer formation, microscopically transparent membranes for easy cell visualization, and fluorescent-compatible plastics and membranes. The 24-well Millicell cell culture plate has a large membrane surface area. The plates are automation compatible and reduce the risk of monolayer contamination with “footed” membrane plates that remain elevated when disassembled from the feeder tray and raised-well edges for better tape seating. Teardrop-shaped receiver wells eliminate air bubbles as plates are assembled. Hanging Millicell cell culture inserts are available in 6-, 12- and 24-well sizes. Low throughput assays can be performed in the single-well unit. Design features include an off-center insert for easier media addition and removal.