ibiTreat surface modification facilitates cell adhesion on μ-Slides. μ-Slides allow cell culturing and high resolution microscopy with one and the same slide. The surface allows microscopic analysis of living and fixed cells, including analysis of endothelial cells, even under perfusion conditions. The adhesion of cells to these coated slides is strong enough to perform flow experiments simulating the physiological shear stress of the blood flow in the abdominal aorta.