SMARTvector shRNA Lentiviral Particles deliver stably-expressed silencing sequences in a broad range of cell types, including those that resist standard transfection such as primary cells, non-dividing cells, stem cells and neuronal cell lines. Thermo Fisher’s Dharmacon scientists applied their groundbreaking research and innovations in siRNA design to develop an shRNA (short-hairpin RNA) design algorithm capable of routinely producing highly functional sequences. This first-ever algorithm is used to design shRNA silencing sequences specific to every gene in the human, mouse and rat genomes. The highly concentrated and purified viral delivery vehicle enables a broad range of experiments and reduces adverse effects on the cell. It produces lentiviral particles at titers of 10⁸ transducing units per milliliter or greater. High titers allow a wide array of options in experimental design and investigation.