Ligate-IT™ Rapid Ligation Kit provides ligation in 5 minutes for cohesive ends and 10 minutes for blunt ends. Competents cells can be transformed immediately, or the ligation reaction may be stored at -20°C for later transformation. The kit includes USB T4 DNA Ligase, Nuclease-Free Water, and a specially formulated 5× Reaction Buffer that enhances the activity of T4 DNA Ligase, especially on blunt-end fragments. All of the components are supplied in a convenient, ready-to-use format and do not require any additional reagents, such as ATP. The kit is flexible and can be used for a variety of ligation procedures such as conventional vector cloning, TA cloning, linker or adaptor ligation, and library construction.