Biacore T100 protein interaction analysis system is offered for use in primary research and drug discovery. The instrument can analyze up to 384 samples per run, automatically, over a broad kinetic range. Software wizards facilitate assay development, analysis and evaluation of every interaction parameter: kinetics, affinity, specificity, concentration and thermodynamics. The system supports assay development, analysis and evaluation when working with compounds of <1000 Da. Integrated buffer degassing improves robustness when studying interactions at elevated temperatures. Applications include elucidation of disease mechanisms by characterizing native or recombinant protein interactions; selection of antibodies as research tools, assay components or therapeutics by fully defining their interaction behavior; definition of potential drug targets and diagnostic markers; development of assays to characterize proteins e.g. low molecular weight (LMW) compound interactions in drug development; selection of protein therapeutic candidates according to their on/off rates; detection and characterization of immune responses during preclinical and clinical development; characterization of protein therapeutics and implementation of QC methods.