Absolutely RNA™ kits facilitate purification of DNA-free total RNA from tissue and cell samples, including samples from laser microdissection. Because DNA contamination can lead to inaccurate analysis, these kits incorporate an on-column DNA removal step specifically for applications such as RT-PCR, microarray target labeling and QRT-PCR. Each kit contains all necessary reagents for purification of total RNA and is optimized for total RNA purification within a specific range of sample sizes and elution volumes.