BACMAX DNA Purification Kit reliably isolates high-quality BAC DNA, based on a modified alkaline-lysis procedure that typically yields 0.6 to 25 μg of BAC DNA from 1.5- to 100-ml cultures of a single-copy BAC. Selective precipitation steps and the incorporation of the RiboShredder RNase Blend remove contaminants that degrade DNA and interfere with downstream applications. There is no need for columns, resins, or organic extractions. The exceptionally pure BAC DNA can be used directly for sequencing, fingerprinting, PCR, and preparation of shotgun libraries.