SequiTherm EXCEL™ II DNA Sequencing Kit may be used for both cycle sequencing and high-temperature isothermal (non-cycle) sequencing with either labeled primers or internal label incorporation using ³²P, ³³P, or ³⁵S radionuclides. A broad range of templates may be sequenced. SequiTherm EXCEL II DNA Polymerase, included in the kit, improves signal intensities allowing sequencing from low amounts of template. Difficult regions can be resolved, including hairpin loops, regions of high GC or AT content, PCR products, inverted or direct repeats, and homopolymeric stretches. When performing isothermal sequencing reactions, go from template denaturation to gel in as little as 4 minutes with no chemical denaturation or precipitation. As little as 1 μCi of ³⁵S is needed per template for non-cycle sequencing reactions. Read lengths up to 600+ bases are obtainable with Long-Read™ Termination Mixes (provided). Reduced background signal results in clearer, less ambiguous sequences.