Sterile disposable Erlenmeyer culture flasks and disposable Erlenmeyer shake flasks are supplied with 2 closures, a solid top closure attached and .2 μm PTFE vented closure. The flasks are graduated, non-pyrogenic and manufactured with Lexan virgin polycarbonate resin. They can be re-autoclaved and re-frozen. The flasks are suited for use with all shaking culture, mixing, storage of biological fluids, media buffers and aqueous solutions.